基于水凝胶微球建立胰腺癌原代细胞的3D培养模型

马芳芳, 秦洁洁, 任灵杰, 唐笑梅, 刘佳, 施敏敏, 蒋玲曦

Establishment of a 3D culture model in vitro of pancreatic cancer primary cells using hydrogel microspheres

MA Fangfang, QIN Jiejie, REN Lingjie, TANG Xiaomei, LIU Jia, SHI Minmin, JIANG Lingxi

图2 正常胰腺细胞和胰腺癌细胞系均能黏附在水凝胶微球表面生长 Note： A. Bright field images of HPNE cells growing on the surface of hydrogel microspheres (×40). B. The number of HPNE cells adhered to the surface of the microspheres and the number of cells suspended in the culture flask were counted. C. Bright field images of 8988 cells growing on the surface of hydrogel microspheres (×40). D. The number of 8988 cells adhered to the surface of the microspheres and the number of cells suspended in the culture flask were counted. E. Fluorescence images of HPNE cells growing on the surface of hydrogel microspheres [DAPI (blue) staining (×100), phalloidin (green) staining (×100), merge (×100, ×200)]. F. Fluorescence images of HPNE cells growing on the surface of hydrogel microspheres [DAPI (blue) staining (×100), phalloidin (green) staining (×100), merge (×100, ×200)]. ①P=0.000, ②P=0.001.

Fig 2 Both normal pancreatic cells and pancreatic cancer cell lines can grow on the surface of hydrogel microspheres