基于水凝胶微球建立胰腺癌原代细胞的3D培养模型

马芳芳, 秦洁洁, 任灵杰, 唐笑梅, 刘佳, 施敏敏, 蒋玲曦

Establishment of a 3D culture model in vitro of pancreatic cancer primary cells using hydrogel microspheres

MA Fangfang, QIN Jiejie, REN Lingjie, TANG Xiaomei, LIU Jia, SHI Minmin, JIANG Lingxi

图3 胰腺癌组织来源细胞能在水凝胶微球表面生长 Note： A. Schematic diagram of the 3D model of pancreatic cancer primary cells based on hydrogel microspheres. B. Bright field image of a 3D model of pancreatic cancer primary cells (×40). C. The numbers of cells adhered to the surface of the microspheres and suspended in the culture flask were counted. D. Fluorescence images of cells growing on the surface of hydrogel microspheres [DAPI (blue) staining (×100), phalloidin (green) staining (×100), merge (×100, ×200)]. ①P=0.000.

Fig 3 Pancreatic cancer tissue-derived cells can grow on the surface of hydrogel microspheres