基于水凝胶微球建立胰腺癌原代细胞的3D培养模型

马芳芳, 秦洁洁, 任灵杰, 唐笑梅, 刘佳, 施敏敏, 蒋玲曦

Establishment of a 3D culture model in vitro of pancreatic cancer primary cells using hydrogel microspheres

MA Fangfang, QIN Jiejie, REN Lingjie, TANG Xiaomei, LIU Jia, SHI Minmin, JIANG Lingxi

图4 胰腺癌原代细胞3D培养模型包含胰腺癌肿瘤微环境的主要细胞成分 Note： A. H-E staining pictures of pancreatic cancer tissue sections (×50). B. The percentage of each cell in the tissue. C. Bright field images of pancreatic cancer primary cell 3D culture model (×100). D. The percentage of each cell in the 3D culture model. E?H. Immunofluorescence staining pictures (×100) of pancreatic cancer tissue sections, stained with CD31, SMA, CD133, EpCAM (red), CK19 (green), and DAPI (blue), respectively. I?L. Immunofluorescence staining pictures (×100) of pancreatic cancer primary cell 3D culture model [confocal microscope x, y axis scan synthetic 2D image stained with CD31, SMA, CD133, EpCAM (red), CK19 (green), and DAPI (blue), respectively]. M?P. Immunofluorescence-stained 3D images (×200) of pancreatic cancer primary cell 3D culture model [confocal microscopy x, y, z axis scans of the synthesized 3D structures, stained with CD31, SMA, CD133, EpCAM (red), CK19 (green), and DAPI (blue), respectively].

Fig 4 Pancreatic cancer primary cell 3D culture model contains major cellular components of the pancreatic cancer tumor microenvironment