淫羊藿苷对急性肾损伤向慢性肾脏病转化小鼠模型的预防性保护作用

谢林, 程烨, 郑琦敏, 张熙, 付莉莉, 陈敏, 王怡, 梅长林, 谢静远, 顾向晨

Preventive effect of icariin on transition from acute kidney injury to chronic kidney disease in mouse model

XIE Lin, CHENG Ye, ZHENG Qimin, ZHANG Xi, FU Lili, CHEN Min, WANG Yi, MEI Changlin, XIE Jingyuan, GU Xiangchen

图9 敲低 Ppara 后淫羊藿苷对肾小管上皮细胞脂肪酸氧化代谢相关基因及凋亡蛋白表达水平的影响 Note： A. Western blotting analysis of PPARα protein expression in epithelial cells after siRNAs intervention. B. Relative mRNA expression level of Ppara in epithelial cells after siRNA3 intervention. C?E. Relative mRNA expression levels of Ppara (C), Cpt1a (D)and Cpt2 (E) in epithelial cells with NC-siRNA or siRNA3 intervention. F. Western blotting analysis of cleaved caspase-3 protein expression in epithelial cells with NC-siRNA or siRNA3 intervention. Each experiment was repeated three times. n=3. ①P=0.027, ②P=0.020, ③P=0.037, ④P=0.060, ⑤P=0.026, ⑥P=0.038, ⑦P=0.046, ⑧P=0.045.

Fig 9 Effect of ICA on expression levels of fatty acid oxidative metabolism-related mRNAs and the apoptotic protein in renal tubular epithelial cells after knocking down Ppara