淫羊藿苷对急性肾损伤向慢性肾脏病转化小鼠模型的预防性保护作用
谢林, 程烨, 郑琦敏, 张熙, 付莉莉, 陈敏, 王怡, 梅长林, 谢静远, 顾向晨

Preventive effect of icariin on transition from acute kidney injury to chronic kidney disease in mouse model
XIE Lin, CHENG Ye, ZHENG Qimin, ZHANG Xi, FU Lili, CHEN Min, WANG Yi, MEI Changlin, XIE Jingyuan, GU Xiangchen
表1 实时荧光定量PCR引物序列
Tab 1 Primer sequences for real-time qPCR
GeneForward (5′→3′)Reward (5′→3′)
Fn (mouse)ATGGTACAGCTGATCCTGCCGCCCTGGTTTGTACCTGCTA
Acta2 (mouse)GGAACCCTGAGACGCTGCTCATTCCAACCATTACTCCC
Col1a1 (mouse)CCCAGCCGCAAAGAGTCTACAGCATACCTCGGGTTTCCAC
Tgfb1 (mouse)GGGAAGCAGTGCCCGAACCCTGGGGGTCAGCAGCCGGTTA
Hprt (mouse)TATGCCGAGGATTTGGAAAATCCCATCTCCTTCATGACATC
Cpt1a (mouse)CTTCCCATTTGACACCTTTGATACGTGAGGCAGAACTTGC
Col3a1 (mouse)ACAGCTGGTGAACCTGGACCAGGAGATCCATCTCGAC
Vimentin (mouse)GGATCAGCTCACCAACGACAGGTCAAGACGTGCCAGAGAA
Cpt2 (mouse)CCCAGGCTGCCTATCCCTTCCTTCCCAATGCCGTTC
Ppara (mouse)AGAGCCCCATCTGTCCTCTCACTGGTAGTCTGCAAAACCAAA
Acox2 (mouse)GAGGGTGAGAATACGGTGGTCTTGGTGTGGCGAG
Ppara (rat)AACATCGAGTGTCGAATATGTGGCCGAATAGTTCGCCGAAAGAA
Tnfa (rat)ATGGGCTCCCTCTCATCAGTGCTTGGTGGTTTGCTACGAC
Fn (rat)AGAGGGGAGTGGAAGTGTGAGTTGTAGGTGAACGGGAGAA
Tgfb1 (rat)CTAATGGTGGACCGCAACAACCACTGCTTCCCGAATGTCTGA
Ctgf (rat)AACCGTGTGTCATTGTCATCACCTTAGTGTGCGTTCTG
Cpt1a (rat)ATGGAGGTTGTCTACGACAGGGGTTTACTTTTTA
Cpt2 (rat)CAGCTGACCAAAGAAGCAGCGTTCAGAGTGCTGGTGGACA
Col1a1 (rat)CTGACGCATGGCCAAGAAGATTGGGTCCCTCGACTCCTAT
β-actin (rat)GTAAAGACCTCTATGCCAACAGGACTCATCGTACTCCTGCT