淫羊藿苷对急性肾损伤向慢性肾脏病转化小鼠模型的预防性保护作用
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谢林, 程烨, 郑琦敏, 张熙, 付莉莉, 陈敏, 王怡, 梅长林, 谢静远, 顾向晨
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Preventive effect of icariin on transition from acute kidney injury to chronic kidney disease in mouse model
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XIE Lin, CHENG Ye, ZHENG Qimin, ZHANG Xi, FU Lili, CHEN Min, WANG Yi, MEI Changlin, XIE Jingyuan, GU Xiangchen
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表1 实时荧光定量PCR引物序列
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Tab 1 Primer sequences for real-time qPCR
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Gene | Forward (5′→3′) | Reward (5′→3′) |
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Fn (mouse) | ATGGTACAGCTGATCCTGCC | GCCCTGGTTTGTACCTGCTA | Acta2 (mouse) | GGAACCCTGAGACGCTGCT | CATTCCAACCATTACTCCC | Col1a1 (mouse) | CCCAGCCGCAAAGAGTCTAC | AGCATACCTCGGGTTTCCAC | Tgfb1 (mouse) | GGGAAGCAGTGCCCGAACCC | TGGGGGTCAGCAGCCGGTTA | Hprt (mouse) | TATGCCGAGGATTTGGAAAA | TCCCATCTCCTTCATGACATC | Cpt1a (mouse) | CTTCCCATTTGACACCTTTG | ATACGTGAGGCAGAACTTGC | Col3a1 (mouse) | ACAGCTGGTGAACCTGG | ACCAGGAGATCCATCTCGAC | Vimentin (mouse) | GGATCAGCTCACCAACGACA | GGTCAAGACGTGCCAGAGAA | Cpt2 (mouse) | CCCAGGCTGCCTATCCCT | TCCTTCCCAATGCCGTTC | Ppara (mouse) | AGAGCCCCATCTGTCCTCTC | ACTGGTAGTCTGCAAAACCAAA | Acox2 (mouse) | GAGGGTGAGAATACGGT | GGTCTTGGTGTGGCGAG | Ppara (rat) | AACATCGAGTGTCGAATATGTGG | CCGAATAGTTCGCCGAAAGAA | Tnfa (rat) | ATGGGCTCCCTCTCATCAGT | GCTTGGTGGTTTGCTACGAC | Fn (rat) | AGAGGGGAGTGGAAGTGTGA | GTTGTAGGTGAACGGGAGAA | Tgfb1 (rat) | CTAATGGTGGACCGCAACAAC | CACTGCTTCCCGAATGTCTGA | Ctgf (rat) | AACCGTGTGTCATTGTCAT | CACCTTAGTGTGCGTTCTG | Cpt1a (rat) | ATGGAGGTTGTCTACGA | CAGGGGTTTACTTTTTA | Cpt2 (rat) | CAGCTGACCAAAGAAGCAGC | GTTCAGAGTGCTGGTGGACA | Col1a1 (rat) | CTGACGCATGGCCAAGAAGA | TTGGGTCCCTCGACTCCTAT | β-actin (rat) | GTAAAGACCTCTATGCCAACA | GGACTCATCGTACTCCTGCT |
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