青少年的成人起病型糖尿病家系NEUROD1基因突变的筛查与功能解析

张娟, 葛晓旭, 张荣, 蒋伏松, 蒋燕燕, 李鸣, 李甜甜, 刘婵薇, 陈亚婷, 刘丽梅

Screening and functional analysis of mutations in NEUROD1 gene in pedigrees of maturity-onset diabetes of the young

ZHANG Juan, GE Xiaoxu, ZHANG Rong, JIANG Fusong, JIANG Yanyan, LI Ming, LI Tiantian, LIU Chanwei, CHEN Yating, LIU Limei

图3 野生型和突变型NeuroD1蛋白的转录活性测定 Note：A. Relative luciferase activities of the human insulin promoter luciferase reporter gene stimulated by mNeuroD1-WT and mNeuroD1-Glu59Gln in MIN6 cells. B. Expression of mNeuroD1-WT-Flag and mNeuroD1-Glu59Gln-Flag by Western blotting assay in MIN6 cells. ①P=0.000,compared with NC; ②P=0.024, compared with mNeuroD1-WT. NC—pcDNA3.3.

Fig 3 Transcriptional activities of wild type and mutant of NeuroD1 protein