PTEN对FoxM1可变剪接的调控及该过程在肿瘤细胞迁移中的作用

王晓玲, 葛梦凯, 沈少明

PTEN-regulated alternative splicing of FoxM1 affects tumor cell migration

WANG Xiaoling, GE Mengkai, SHEN Shaoming

图1 PTEN 敲低细胞内 FoxM1 mRNA亚型的变化 Note： A. Schematic diagram of primer design strategy for mRNA of FoxM1B, FoxM1C and total FoxM1. B. The specificity of the primers was verified by using qRT-PCR in FoxM1B/FoxM1C overexpressing 293T cells. C. Western blotting results showed that PTEN was efficiently knocked down by two pairs of shRNA both in 293T and DU145 cells. D. qRT-PCR results showed that with PTEN knockdown, the expression of FoxM1B mRNA was upregulated in 293T (left) and DU145 (right) cells, whilethe expression of FoxM1C mRNA was downregulated in 293T cells but did not change in DU145 cells. E. qRT-PCR results showed upregulation of FoxM1B mRNA expression, but no significant change in FoxM1C mRNA expression after PTEN being knocked down in RKO (left), SW480 (middle) and SW460 (right) cells. NRD—N-terminal repressor domain; FKH—forkhead/winged-helix domain; TAD—transcativation domain; EV—empty vector.

Fig 1 FoxM1 mRNA isoforms changed in PTEN knockdown cells