PTEN对FoxM1可变剪接的调控及该过程在肿瘤细胞迁移中的作用

王晓玲, 葛梦凯, 沈少明

PTEN-regulated alternative splicing of FoxM1 affects tumor cell migration

WANG Xiaoling, GE Mengkai, SHEN Shaoming

图3 FoxM1B/C转录活性和β-catenin定位鉴定 Note： A. Immunofluorescence of 293T cells transfected with Plvx-FOXM1B-Flag and Plvx-FoxM1C-Flag. DAPI represents nucleus, green represents β-catenin, and red represents Flag. Neither FoxM1B nor FoxM1C could cause β-catenin to enter the nucleus. B. Schematic diagram of double luciferase reporter expression system. C. Protein expression of FoxM1B and FoxM1C in FoxM1B/C overexpressing 293T cells transfected with Firefly luciferase and Renilla luciferase reporter gene plasmids. D. Transcriptional activity of FoxM1B and FoxM1C. There was no significant difference in the relative Luc/Ren ratio between FoxM1B and FoxM1C overexpressing 293T cells.

Fig 3 FoxM1B /C transcriptional activities and β-catenin localization