基于甲型流感病毒H1N1亚型血凝素的mRNA疫苗制备和加强免疫策略研究

沈海浅, 俞康莹, 陈颖盈, 季萍, 王颖

Construction of an mRNA vaccine encoding hemagglutinin of influenza A H1N1 virus and investigation on booster immunization strategy

SHEN Haiqian, YU Kangying, CHEN Yingying, JI Ping, WANG Ying

图1 FLuc mRNA-LNP的制备及鉴定 Note： A. Full length of FLuc mRNA (2 000 nt) determined by gel electrophoresis. M—RNA ladder; lane 1—FLuc mRNA after IVT; lane 2—FLuc mRNA after 5′ capping. B. Luciferase activity detection. C. The particle size of FLuc mRNA-LNP measured by dynamic light scattering method.

Fig 1 Preparation and identification of FLuc mRNA-LNP