基于甲型流感病毒H1N1亚型血凝素的mRNA疫苗制备和加强免疫策略研究

沈海浅, 俞康莹, 陈颖盈, 季萍, 王颖

Construction of an mRNA vaccine encoding hemagglutinin of influenza A H1N1 virus and investigation on booster immunization strategy

SHEN Haiqian, YU Kangying, CHEN Yingying, JI Ping, WANG Ying

图3 M15-HA mRNA-LNP制备及鉴定 Note： A. Full length of M15-HA mRNA (2 100 nt) determined by gel electrophoresis. M—RNA ladder; lane 1—M15-HA mRNA after IVT; lane 2—M15-HA mRNA after 5′ capping. B. Detection of M15-HA protein in HEK293T cells transfected with M15-HA mRNA by Western blotting. M—protein marker; lane 1—the control without transfection; lane 2—the cells transfected with M15-HA mRNA. C. The particle size of M15-HA mRNA-LNP measured by dynamic light scattering method.

Fig 3 Preparation and identification of M15-HA mRNA-LNP