鲍曼不动杆菌在环境美罗培南浓度变化时耐药性的改变及其机制

赵富茂, 彭玫, 彭晓露, 舒韦韦, 彭丽

Changes in drug resistance of Acinetobacter baumannii during the change of meropenem concentration in the environment and its mechanism

ZHAO Fumao, PENG Mei, PENG Xiaolu, SHU Weiwei, PENG Li

表1 碳青霉烯酶基因PCR鉴定引物

Tab 1 Primers used in the identification of carbapenemase genes by PCR

Ambler class Gene Forward primer (5′→3′) Reverse primer (5′→3′) Fragment size/bp Reference A IMI ATAGCCATCCTTGTTTAGCTC TCTGCGATTACTTTATCCTC 818 [12] KPC GCTTCCCACTGTGCAGCTCATTCA CGGTCGTGTTTCCCTTTAGCCAATC 456 － GES-1 ATGCGCTTCATTCACGCAC CTATTTGTCCGTGCTAAGG 814 [13] B IMP AGGCCAAGATCGTTTAGTCACTGT GTGTTGTACAGATAGAACCAGGACCAA 461 － VIM TGGTGAGTATCCGACAGTCAACGA CGAATGCGCAGCACCAG 450 － NDM-1 AGGACAAGATGGGCGGTATGGACG CCATGCGGGCCGTATGAGTGATT 429 － D OXA51 ATGAACATTAAAGCACTC CTATAAAATACCTAATTGTTC 825 [11] OXA23 ACTTGCTATGTGGTTGCTTC TGGAAGCTGTGTATGTGCTA 555 [14] OXA24 TTTGCCGATGACCTTGCACATAAC TCATGTTGAGCGAAAAGGGGATTTTT 208 － OXA58 CGATCAGAATGTTCAAGCGC TCCCCTCTGCGCTCTACATACA 666 －