鲍曼不动杆菌在环境美罗培南浓度变化时耐药性的改变及其机制
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赵富茂, 彭玫, 彭晓露, 舒韦韦, 彭丽
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Changes in drug resistance of Acinetobacter baumannii during the change of meropenem concentration in the environment and its mechanism
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ZHAO Fumao, PENG Mei, PENG Xiaolu, SHU Weiwei, PENG Li
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表2 RT-qPCR引物
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Tab 2 Primers used in RT-qPCR analysis
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Gene | Forward primer (5′→3′) | Reverse primer (5′→3′) | Reference |
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16S rRNA | GTAGCTTGCTACTGGACCTAG | CATACTCTAGCTCACCAGTATCG | [15] | OXA51 | GATTTAGCTCGTCGTATTGGA | AAGCGTTTTATTAGCTAGCTTG | [16] | adeB | GGAATAAGGCACCACAACAAT | CGAAGTTAGGAATACCAGCAATAC | - | adeG | TCACCAGATAATCGCTATG | GACTTCACCTACACCTTG | - | adeJ | CCTATTGCACAATATCCAACGA | AGGATAAGTCGCAGCAATCG | [17] | oprC | ACTCGATACAAAGCGGTGGA | TTTAATACGTGAACCAAACATACCTC | [17] | carO | TGTTCATGACAGCTATGCATTCGATA | CCCAATGCTAAACCTACATATGGGT | [17] | omp33-36 | GCAACTTACAACCACACTGA | TAACAACATAGCACCAACTTCTAA | - | ponA | GTCAGCCAGGTTCTACCATCAA | CCATCAGAGTTCTTCGGTGTCC | - |
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