FBXO38通过PI3K-Akt信号通路调控眼部黑色素瘤增殖

吴以加, 房燕, 沈菲洋, 黄蕊, 沈键锋, 范先群

FBXO38 regulates ocular melanoma proliferation through the PI3K-Akt signaling pathway

WU Yijia, FANG Yan, SHEN Feiyang, HUANG Rui, SHEN Jianfeng, FAN Xianqun

图2 FBXO38 敲低或者过表达对黑色素瘤细胞增殖的影响 Note： A. Representative images of colony formation in FBXO38 knockdown and overexpression A375 cells. B. Colony formation efficiency of A375 cell lines after 7-d incubation. C. Representative images of colony formation on FBXO38 knockdown and overexpression OMM2.3 cells. D. Colony formation efficiency of OMM2.3 cell lines after 7-d incubation. E. Immunofluorescence staining of BrdU in FBXO38 knockdown and overexpression A375 cells. Scale bars, 50 μm. F. The percentage of BrdU positive A375 cells. G/H. Cell viability of FBXO38 knockdown and overexpression A375 cells (G) and OMM2.3 cells (H) detected by CCK8 assay. ①P=0.046, ②P=0.048, ③P=0.039, ④P=0.001, ⑤P=0.003, ⑥P=0.010, ⑦P=0.012, ⑧P=0.036, ⑨P=0.024, ⑩P=0.033, ?P=0.005, ?P=0.016, ?P=0.017.

Fig 2 Effect of FBXO38 knockdown or overexpression on the proliferation of melanoma cells