FBXO38通过PI3K-Akt信号通路调控眼部黑色素瘤增殖

吴以加, 房燕, 沈菲洋, 黄蕊, 沈键锋, 范先群

FBXO38 regulates ocular melanoma proliferation through the PI3K-Akt signaling pathway

WU Yijia, FANG Yan, SHEN Feiyang, HUANG Rui, SHEN Jianfeng, FAN Xianqun

图4 FBXO38 对PI3K-Akt信号通路和下游分子的调节 Note： A/B. Detection of inhibition rate of different concentration of LY294002 (A) or Everolimus (B) on A375 cells after 24 h of treatment by using CCK8 assay. C/D. Detection of inhibition rate of different concentration of LY294002 (C) or Everolimus (D) on OMM2.3 cells after 24 h of treatment by using CCK8 assay. E/F. Expression of p-PI3K, p-Akt, p-mTOR, PTEN, MDM2, P53 and P21 in FBXO38 knockdown and control A375 cells (E) and OMM2.3 cells (F) detected by Western blotting. G/H. Transcription level of TP53 and MDM2 in FBXO38 knockdown and control A375 cells (G) and OMM2.3 cells (H) detected by qRT-PCR. ①P=0.003, ②P=0.030, ③P=0.044, ④P=0.006, ⑤P=0.013, ⑥P=0.001, ⑦P=0.041, ⑧P=0.017, ⑨P=0.012, ⑩P=0.004, ?P=0.011, ?P=0.027, ?P=0.015, ?P=0.006, ?P=0.014, ?P=0.033, 17P=0.040, 18P=0.048, 19P=0.005, 20P=0.020, 21P=0.042, 22P=0.024, 23P=0.008, 24P=0.023, 25P=0.010, 26P=0.002.

Fig 4 Regulation of FBXO38 on the PI3K-Akt signaling pathway and downstream molecular