腹腔灌洗液循环肿瘤DNA在预测胃肠道恶性肿瘤腹膜转移中应用的研究进展

白龙, 夏翔, 曹晖, 张子臻

Progress in application of peritoneal lavage fluid circulating tumor DNA to predicting peritoneal metastasis of gastrointestinal cancer

BAI Long, XIA Xiang, CAO Hui, ZHANG Zizhen

表1 胃癌患者腹腔灌洗液中基因甲基化检测相关研究

Tab 1 Detection of gene methylation in peritoneal lavage fluid in gastric cancer patients

Literature	No. of patients	Detecting technique	Target site of DNA	Positive result in PLF/%(n)	Prediction of peritoneal recurrence
HIRAKI^[45]	80	Real-time methylation-specific PCR	CHFR, E-cadherin, BNIP3	19% (6/31)^①	2 of 5 patients with multigene methylation showed peritoneal recurrence after surgery
HIRAKI^[46]	107	Real-time methylation-specific PCR	BNIP3,CHFR,CYP1B1,MINT25,RASSF2,SFRP2	20% (9/45)^②	3 of 9 patients carrying positive methylation experienced peritoneal recurrence
YU^[47]	92	Real-time methylation-specific PCR	CDH1 methylation	48.9% (45/92)	CDH1 methylation correlated significantly with distant metastasis (P<0.005)
HAN^[48]	92	Real-time methylation-specific PCR	Methylated MINT2	90.2% (37/41)	Levels of methylated MINT2 DNA in PLF was significantly different between patients with and without peritoneal metastasis (P<0.000 1)
YU^[49]	92	Real-time methylation-specific PCR	TIMP-3 methylation	53.3% (49/92)	TIMP-3 hypermethylation in PLF (γ=0.804, P<0.001) was closely correlated with positive PLF cytology
HU^[50]	92	Quantitative methylation-specific PCR	Methylated THBS1	58.7% (54/92)	Accuracy 63.0% (34/54), Sensitivity 87.2% (34/39), Specificity 100% (34/34)