分选链接蛋白1在胰腺导管腺癌中的表达及其促进胰腺导管腺癌进展的机制研究

潘泓, 廖颖娜, 盖严支, 钱立恒, 聂惠贞

Expression of sorting nexin 1 in pancreatic ductal adenocarcinoma and its mechanism in promoting PDAC progress

PAN Hong, LIAO Yingna, GAI Yanzhi, QIAN Liheng, NIE Huizhen

图2 体内、外研究分析下调 SNX1 对PDAC细胞增殖能力的影响 Note： A. Detection of SNX1 expression in AsPC-1 and Capan-1 cells by Western blotting. B/C. Detection of the effect of SNX1 knockdown on the proliferation capacity of AsPC-1 and Capan-1 cells by CCK8 (B) and plate clonal formation experiment (C). ①P=0.000, ②P=0.002, ③P=0.008, compared with the NC-siRNA group. D. Detection of SNX1 expression in Capan-1 cells by Western blotting. E. Photos of subcutaneous xenograft tumors from Capan-1 cells infected with NC-shRNA or SNX1-shRNA lentivirus. F. Statistical analysis of tumor weight growth of Capan-1 cells infected with NC-shRNA or SNX1-shRNA lentivirus over 3 weeks. ④P=0.000, compared with the NC-shRNA group.

Fig 2 Effect of SNX1 knockdown on the proliferation capacity of PDAC cells in vivo and in vitro