基于CRISPR/Cas9n技术建立携带mT-F2A-EGFP报告系统的小鼠胚胎干细胞系

王靖怡, 王琼

Establishment of a mouse embryonic stem cell line carrying a reporter of mT-F2A-EGFP based on CRISPR/Cas9n technology

WANG Jingyi, WANG Qiong

图1 pX461-mT-sgRNA质粒和mT-F2A-EGFP-Donor质粒的构建 Note：A. Scheme of pX461-mT-sgRNA plasmid map. B. Sequencing result of mT-sgRNA cloned into pX461-Cas9n-2A-Hygro plasmid. C. Scheme of mT-F2A-EGFP-Donor plasmid map.

Fig 1 Construction of pX461-mT-sgRNA plasmid and mT-F2A-EGFP-Donor plasmid