基于CRISPR/Cas9n技术建立携带mT-F2A-EGFP报告系统的小鼠胚胎干细胞系

王靖怡, 王琼

Establishment of a mouse embryonic stem cell line carrying a reporter of mT-F2A-EGFP based on CRISPR/Cas9n technology

WANG Jingyi, WANG Qiong

图2 共转染pX461-mT-sgRNA和mT-F2A-EGFP-Donor质粒 Note： A. The scheme showed that F2A-EGFP was inserted before the stop codon of T gene. B. The cell morphology of E14 cells 24 h after electroporation with 2 kinds of plasmids (upper left), 3 d after Hygro and blasticidin selection (middle upper) and another 3 d with blasticidin (upper right). The untransfected E14 cells were used as a control (bottom).

Fig 2 Co-transfection of pX461-mT-sgRNA and mT-F2A-EGFP-Donor plasmid