基于CRISPR/Cas9n技术建立携带mT-F2A-EGFP报告系统的小鼠胚胎干细胞系

王靖怡, 王琼

Establishment of a mouse embryonic stem cell line carrying a reporter of mT-F2A-EGFP based on CRISPR/Cas9n technology

WANG Jingyi, WANG Qiong

图3 mT-F2A-EGFP单克隆细胞的鉴定 Note：A. Gel electrophoresis of PCR products with primers of T-HAL-F/T-EGFP-R (left, 1 596 bp ) or T-BSD-F/T-HAL-R (right, 1 435 bp). B. Gel electrophoresis of PCR products with primers of mT-F/mT-R (3 253 bp). C. Sequencing results of T1, T2, T3, T4 and T5 clones. Primers used for PCR were also labeled here. PC—positive control; NC—negative control; M—marker.

Fig 3 Identification of mT-F2A-EGFP clones