基于CRISPR/Cas9n技术建立携带mT-F2A-EGFP报告系统的小鼠胚胎干细胞系

王靖怡, 王琼

Establishment of a mouse embryonic stem cell line carrying a reporter of mT-F2A-EGFP based on CRISPR/Cas9n technology

WANG Jingyi, WANG Qiong

图4 mT-F2A-EGFP单克隆细胞分化过程中各标记基因的相对表达量 Note： RT-qPCR experiments show relative mRNA levels of pluripotent marker (Sox2), mesendoderm marker (T, Eomes and Gsc), and ectoderm marker (nestin). RNA of E14, T1, T2, T3, T4 and T5 clones were collected before differentiation (D0), and on the 3rd (D3) and the 4th (D4) day of EB differentiation.

Fig 4 Relative expression levels of marker genes during lineage differentiation of mT-F2A-EGFP clones