基于CRISPR/Cas9n技术建立携带mT-F2A-EGFP报告系统的小鼠胚胎干细胞系

王靖怡, 王琼

Establishment of a mouse embryonic stem cell line carrying a reporter of mT-F2A-EGFP based on CRISPR/Cas9n technology

WANG Jingyi, WANG Qiong

图6 应用mT-F2A-EGFP报告系统分选中内胚层细胞 Note：A. Flow cytometry analysis of E14 and T1 on EB differentiation day 4. Pink represents EGFP＋ cells, and red represents EGFP- cells. B. Both EGFP＋ and EGFP- cells of T1 on EB differentiation day 4 were collected by flow cytometry for RNA extraction. Relative mRNA levels of pluripotent marker Sox2, ectoderm markers Pax6 and Sox1, 2CLC markers Dux, Zscan4d and Zscan4, and mesendoderm markers T, Eomes and Gsc of EGFP＋ and EGFP- cells were detected by RT-qPCR. ① P=0.000, ②P=0.005, compared with the EGFP- group.

Fig 6 mT-F2A-EGFP fluorescent reporter stem cell line used to sort mesendoderm cell populations