基于CRISPR/Cas9n技术建立携带mT-F2A-EGFP报告系统的小鼠胚胎干细胞系
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王靖怡, 王琼
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Establishment of a mouse embryonic stem cell line carrying a reporter of mT-F2A-EGFP based on CRISPR/Cas9n technology
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WANG Jingyi, WANG Qiong
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表1 RT-qPCR引物序列
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Tab 1 Primer sequences for RT-qPCR
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| Gene | Forward primer (5'→3') | Reverse primer (5'→3') |
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| Gapdh | CTCCACTCACGGCAAATTCA | CGCTCCTGGAAGATGGTGAT | | Sox2 | GCGGAGTGGAAACTTTTGTCC | CGGGAAGCGTGTACTTATCCTT | | Gsc | TTGCACAGACAGTCGATGCTACT | TCGTTGCTTTCTCGACCCC | | T | TCCTCCATGTGCTGAGACTTGT | CCAAGAGCCTGCCACTTTG | | Eomes | GCGCATGTTTCCTTTCTTGAG | GGTCGGCCAGAACCACTTC | | nestin | CAGGATTGGGAGGAGGGCAGAG | GGAGGCAGGAGACTTCAGGTAG | | Pax6 | GTTCCCTGTCCTGTGGACTC | ACCGCCCTTGGTTAAAGTCT | | Sox1 | ATACCGCAATCCCCTCTCAG | ACAACATCCGACTCCTCTTCC | | Dux | CCCAGCGACTCAAACTCCTTC | GGACTTCGTCCAGCAGTTGAT | | Zscan4 | GAGATTCATGGAGAGTCTGACTGATGAGTG | GCTGTTGTTTCAAAAGCTTGATGACTTC | | Zscan4d | GTCCTGACAGAGGCCTGCC | GAGATGTCTGAAGAGGCAAT |
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