蓝藻丝氨酸蛋白酶抑制剂arthropin的制备及其靶蛋白酶的筛选

许佳伟, 周爱武, 杨愈丰

Preparation and target protease identification of a cyanobacterial serine protease inhibitor, arthropin

XU Jiawei, ZHOU Aiwu, YANG Yufeng

图3 Arthropin靶蛋白酶的筛选与反应动力学检测 Note：A. Arthropin (3 μg) was incubated with the proteases (0.4 μg), respectively, and the complex formation was analyzed by SDS-PAGE. The red arrows indicate the complexes of arthropin with the target proteases. M—marker; Try—trypsin; Arth—arthropin; Cpx—arthropin-proteinase covalent complex. B. Determination of the SI (left panel) and the kapp (right panel) of arthropin's inhibition of KLK1.

Fig 3 Screening and reaction kinetics assays for target proteases of arthropin