蓝藻丝氨酸蛋白酶抑制剂arthropin的制备及其靶蛋白酶的筛选

许佳伟, 周爱武, 杨愈丰

Preparation and target protease identification of a cyanobacterial serine protease inhibitor, arthropin

XU Jiawei, ZHOU Aiwu, YANG Yufeng

图4 不同结晶条件筛选得到的arthropin蛋白质晶体(×6.3) Note：A. 25% PEG MME 550, 0.1 mol/L MES (pH 6.5), and 0.01 mol/L ZnCl2. B. 20% PEG 4000, 20% iso-propanol, and 0.1 mol/L sodium citrate (pH 5.6). C. 0.4 mol/L NaH2PO4, 1.6 mol/L K2HPO4, 0.1 mol/L imidazole (pH 8.0), and 0.2 mol/L NaCl (final pH 7.6).

Fig 4 Arthropin crystals from different crystallization conditions (×6.3)