靶向抑制CDK12/13在高级别胶质瘤中的体外治疗效果和作用分子机制探究

梅艳青, 韩雨洁, 翁文筠, 张蕾, 唐玉杰

In vitro therapeutic effects and molecular mechanisms of targeted inhibition of CDK12/13 in high-grade gliomas

MEI Yanqing, HAN Yujie, WENG Wenyun, ZHANG Lei, TANG Yujie

图4 靶向抑制CDK12/13显著降低GBM和DIPG细胞DDR相关基因的富集 Note： A. Venn diagram showing differentially expressed genes (Gfold<-1) of 0.1 μmol/L or 1 μmol/L SR-4835 versus DMSO-treated DIPG17 and SF188 cell lines. B. GO analysis on enriched function of down-regulated genes in DIPG17 and SF188 cell lines treated with 0.1 μmol/L or 1 μmol/L SR-4835. C. GSVA analysis of enriched gene sets in DIPG17 and SF188 cell lines. D. Heatmap analysis of RNA-seq data showing transcript level expression alteration of representative DDR-related genes after SR-4835 treatment.

Fig 4 Targeted inhibition of CDK12/13 significantly reduced the enrichment of gene sets involved in DDR in GBM and DIPG cells