靶向抑制CDK12/13在高级别胶质瘤中的体外治疗效果和作用分子机制探究

梅艳青, 韩雨洁, 翁文筠, 张蕾, 唐玉杰

In vitro therapeutic effects and molecular mechanisms of targeted inhibition of CDK12/13 in high-grade gliomas

MEI Yanqing, HAN Yujie, WENG Wenyun, ZHANG Lei, TANG Yujie

表2 RT-qPCR引物序列

Tab 2 Primer sequence for RT-qPCR

Primer Forward (5'→3') Reverse (5'→3') ATM GCTGACAATCATCACCAAGT GGTTCTCAGCACTATGGGACA ATR CGCTGAACTGTACGTGGAAA CAATTAGTGCCTGGTGAACATC BRCA1 CTGCTCAGGGCTATCCTCTCA GCTTCTAGTTCAGCCATTTCCTG CCNK AAAGCCATGTTGGTACTGGGA TGTAGCCCCAAACGTGTGC E2F1 CATCAGTACCTGGCCGAGAG CCCGGGGATTTCACACCTTT FANCD2 CCCAGAACTGATCAACTCTCCT CCATCATCACACGGAAGAAA FANCI CACCACACTTACAGCCCTTG ATTCCTCCGGAGCTCTGAC RAD51 GCTGATGAGTTTGGTGTAGCAG GGAAGACAGGGAGAGTCGTAGA SMARCC2 GAGGGATAAGCAGGTTCTTCTG GGGATCCACGTGTCGTAACT TP53 CCCAAGCAATGGATGATTTGA GCATTCTGGGAGCTTCATCT GAPDH TGACTTCAACAGCGACACCCA CACCCTGTTGCTGTAGCCAAA dGAPDH CGTTCATGCCACCACCGCTA CCACGTCCATCACGCCACAA