孟鲁司特对哮喘中白三烯B4代谢的影响

胡煜, 谢亮, 邹丹, 伏洪玲, 娄丽丽, 谢柯祺, 刘瀚旻

Effect of montelukast on leukotriene B4 metabolism in asthma

HU Yu, XIE Liang, ZOU Dan, FU Hongling, LOU Lili, XIE Keqi, LIU Hanmin

图8 OVA再次激发哮喘小鼠后 ALOX5AP 、 LTA4H 和 BLT1 基因及蛋白表达 Note： A. RT-qPCR transcription levels of ALOX5AP, LTA4H and BLT1 gene of control, AAD, AAD+montelukast and AAD+montelukast-NC mice (n=4). B. Immunohistochemical staining of ALOX5AP, LTA4H and BLT1 protein lung samples (×400) and the IOD values of each protein in immunohistochemical staining (n=8). C. Western blotting electrophoresis bands of ALOX5AP, LTA4H and BLT1 protein and the ratio values of each protein (n=4). ①P=0.000, ②P=0.007, ③P=0.011, ④P=0.012, ⑤P=0.009, ⑥P=0.017.

Fig 8 Transcription and protein levels of ALOX5AP, LTA4H and BLT1 genes after re-challenged by OVA in asthma mice models