miR-185-5p通过抑制巨噬细胞自噬促进胞内分枝杆菌生长

吴淇琦, 汪豪, 林砺, 晏博, 张舒林

miR-185-5p facilitates intracellular Mycobacterium growth via inhibiting macrophage autophagy

WU Qiqi, WANG Hao, LIN Li, YAN Bo, ZHANG Shulin

图1 外泌体鉴定、摄取及BCG感染对巨噬细胞和外泌体中miR-185-5p表达水平的影响 Note：A. Western blotting analysis of the expression levels of exosome marker protein CD9 and TSG101. Ctrl—uninfected macrophages. B. Detection of exosome diameter and concentration by NTA. C. TEM analysis of exosome characteristics. D. The relative expression level of miR-185-5p in exosomes from macrophages with BCG infection for 48 h. E. The relative expression level of miR-185-5p in macrophages with BCG infection for 0, 12, 24 and 48 h. F. Cellular uptake of exosomes extracted from BCG-infected macrophages by uninfected macrophages after 0, 12, 24 and 48 h of co-culture. ①P=0.000.

Fig 1 Identification and uptake of exosomes and the effect of BCG infection on the expression of miR-185-5p