miR-185-5p通过抑制巨噬细胞自噬促进胞内分枝杆菌生长

吴淇琦, 汪豪, 林砺, 晏博, 张舒林

miR-185-5p facilitates intracellular Mycobacterium growth via inhibiting macrophage autophagy

WU Qiqi, WANG Hao, LIN Li, YAN Bo, ZHANG Shulin

图3 miR-185-5p对巨噬细胞自噬流的影响 Note：A–C. Western blotting analysis of autophagy protein LC3 Ⅱ in macrophages. A. THP-1-derived macrophages were treated with inhibitor/inhibitor NC with or without BCG infection. B. THP-1-derived macrophages were treated with mimic/mimic NC with or without BCG infection. C. THP-1-derived macrophages were transfected with miR-185-5p inhibitor followed by BCG infection and CQ treatment (10 μmol/L). D. Confocal images of LC3 protein. GFP (green) and RFP (red) indicate the aggregation of LC3 protein in autophagosomes and autophagolysosomes, respectively. E. Quantification of RFP and GFP fluorescent puncta. ①P=0.034, ②P=0.042.

Fig 3 Effect of miR-185-5p on macrophage autophagic flux