轻链单个氨基酸改变可增强新冠病毒嵌合抗体的中和能力
殷姿, 廉朝阳, 高波, 田莹, 郝茜, 叶菱秀

Enhancement of the neutralization ability resulting from a single amino acid change in the light chain of a chimeric antibody against SARS-CoV-2
YIN Zi, LIAN Chaoyang, GAO Bo, TIAN Ying, HAO Qian, YEAP Lengsiew
表 1 鼠源抗体轻链基因文库构建的第一轮巢式PCR的引物序列
Tab 1 Primer sequences of the first-round nested PCR for constructing mouse-origin antibody light chain gene library
PrimerSequence (5'→3')
1mFK_ⅠRGTGCAGATTTTCAGCTTCCTGCT
1mFK_ⅡTGGACATGAGGGCYCCTGCTCAGT
1mFK_ⅢCTSTGGTTGTCTGGTGTTGAYGGA
1mFK_ⅣGTTGCTGCTGCTGTGGCTTACA
1mFK_ⅤGTATCTGGTACCTGTGG
1mFK_ⅥTGCCTGTTAGGCTGTTGGTGCT
1mFK_ⅦGCTCAGTTCCTTGGTCTCCTGTTGC
1mFK_ⅧTGGGTGCTGCTGCTCTGGGT
1mFK_ⅨCAGTTCCTGTTTCTGTTARTGCTCTGG
1mFK_ⅩTGCTCTGGTTATATGGTGCTGATGGG
1mFK_ⅪGCTGTTTTGTATACCTGGG
1mFK_ⅫCTCAGCTCCTGTTGCTGTGGC
1mFK_􀃼CCTCATATTTTTGCTGCTATGGG
1mFK_ⅩⅣTGCTTTTCTGGATTTCAGCCTCCAG
1mFK_ⅩⅤTCAACTTCTGCTCTTCCTGCTGTTC
1mFK_ⅩⅥCTAGCTCYTCTCCTCAGYCTTCTTCTCCTC
1mFK_ⅩⅦGTGTMTGGTGCTBRTGGG
1mFK_ⅩⅧSTGYTGHTGYTCTGG
1mFK_ⅩⅨGGGTATCTGGTACCTGTGG
1mRKACTGAGGCACCTCCAGATGTT
1mFL_ⅠACTTATACTCTCTCTCCTGGCTCTC
1mFL_ⅡCTCTTCTTCTTCTTTGTTCTTCATTGCT
1mRLGTACCATYTGCCTTCCAGKCCACT