轻链单个氨基酸改变可增强新冠病毒嵌合抗体的中和能力
殷姿, 廉朝阳, 高波, 田莹, 郝茜, 叶菱秀

Enhancement of the neutralization ability resulting from a single amino acid change in the light chain of a chimeric antibody against SARS-CoV-2
YIN Zi, LIAN Chaoyang, GAO Bo, TIAN Ying, HAO Qian, YEAP Lengsiew
表 3 鼠源抗体轻链单个氨基酸改变的质粒构建的引物序列
Tab 3 Primer sequences for plasmid construction with single amino acid mutation in mouse-origin antibody light chain
PrimerSequence (5 ́→3 ́)
Adapter forward primerTTCCCAGGTTCCAGATGCCAGGCTGTTGTGACTCAG
F98-deletion reverse primerTGACCTTGGTTCCTCCACCGAACACCCAATGGTTGCTGTA
T76-mutation reverse primerCCTTGGTTCCTCCACCGAACACCCAGAAATGGTTGCTGTACCATAG
Adapter reverse primerGGTTGGCCTTGGGCTGACCTAGGACAGTGACCTTGG