水凝胶刚度影响髓核细胞表型及其功能的体内外研究

陈泽昊, 吕振东, 张震, 崔文国, 张煜辉

Effect of hydrogel stiffness on nucleus pulposus cell phenotypes in vitro and its repairment of intervertebral disc in vivo

CHEN Zehao, LÜ Zhendong, ZHANG Zhen, CUI Wenguo, ZHANG Yuhui

图3 在不同刚度水凝胶基质上培养的髓核细胞形态观察 Note： A. Cell morphology of the soft hydrogel group. B. Cell morphology of the stiff hydrogel group. C. Immunofluorescent image of F-actin in nucleus pulposus cells of the soft hydrogel group. D. Immunofluorescent image of F-actin in nucleus pulposus cells of the stiff hydrogel group. E. Ratio of cell elongation of different groups. F. Quantitative results of cell spreading area cultured on different hydrogels. ①P=0.002, ②P=0.000, compared with the soft hydrogel group.

Fig 3 Morphologic observation of nucleus pulposus cells cultured on soft and stiff hydrogel mediums