水凝胶刚度影响髓核细胞表型及其功能的体内外研究

陈泽昊, 吕振东, 张震, 崔文国, 张煜辉

Effect of hydrogel stiffness on nucleus pulposus cell phenotypes in vitro and its repairment of intervertebral disc in vivo

CHEN Zehao, LÜ Zhendong, ZHANG Zhen, CUI Wenguo, ZHANG Yuhui

表1 qPCR引物序列

Tab 1 Primer sequences for qPCR

Gene Primer sequence (5′→3′) Sox9 Forward AGCACAAGAAAGACCACCCC Reverse GACCCTGAGATTGCCCGGAG Acan Forward GACCTGTGTGAGATCGACCA Reverse GTTGGTTTGGACGCCACTTC Ncam-1 Forward TGGTCAAGTACAGAGCGAAGC Reverse AGGGACTTGAGCATGACGTG Gapdh Forward GACAGCCGCATCTTCTTGTG Reverse ATCCGTTCACACCGACCTTC