AAK1相互作用蛋白的筛选及其调控细胞内整体翻译水平的研究
Screening of AAK1 interaction proteins and its role in regulating global translation level in cells
Note： A.AAK1-3xFLAG and FMRP-MYC formed a complex in HEK-293T cells, detected by CoIP assay by using anti-FLAG beads, followed by immunoblotting with anti-MYC and anti-FLAG antibodies. B. Endogenous AAK1 and FMRP formed a complex in HEK-293T cells, as demonstrated by CoIP assay by using anti-IgG or anti-AAK1, followed by immunoblotting with anti-FMRP or anti-AAK1 antibodies. C. EGFP-tagged AAK1 co-localized with mCherry-tagged FMRP in cytoplasm of Hela cells. Hela cells were co-transfected with plasmids encoding mCherry-FMRP and EGFP-AAK1. The nuclear of cells was visualized with DAPI staining, and then the fluorescence were detected by confocal microscopy. D. GST Pulldown assays indicated that FMRP protein interacted directly with HIS6-tagged AAK1. PD—GST Pulldown.
