脑卒中后脑血管内皮细胞内质网应激抑制Wnt7/β-catenin通路导致血脑屏障损伤的机制研究

董海平, 谢海怡, 马晓晓, 王震虹

Mechanism of blood-brain barrier damage caused by the inhibition of Wnt7/β-catenin pathway induced by endoplasmic reticulum stress in cerebrovascular endothelial cells after stroke

DONG Haiping, XIE Haiyi, MA Xiaoxiao, WANG Zhenhong

图2 OGD引起的HBMEC损伤以及抑制内质网应激对该损伤的缓解作用 Note： A. Determination of activity of HBMECs by CCK-8 assay. B. The cell permeability of HBMECs analyzed with FITC-BSA leakage. C. Determination of the PDGF-β secretion of HBMECs by ELISA. D. Determination of the expression of ZO-1 and claudin-5 in HBMECs by Western blotting. ①P=0.000, ③P=0.002, ⑤P=0.001, compared with the Blank group; ②P=0.026, ④P=0.005, ⑥P=0.042, ⑦P=0.000, ⑧P=0.001, compared with the OGD group.

Fig 2 OGD-induced HBMEC damage and the alleviating effect of inhibiting endoplasmic reticulum stress on this injury