二甲双胍改善由C9ORF72肌萎缩侧索硬化/额颞叶痴呆相关多聚甘氨酸-精氨酸诱导的线粒体损伤

冯奕源, 徐忠匀, 尹雅芙, 王辉, 程维维

Metformin ameliorates the mitochondrial damage induced by C9ORF72 amyotrophic lateral sclerosis/frontotemporal dementia-related poly-GR

FENG Yiyuan, XU Zhongyun, YIN Yafu, WANG Hui, CHENG Weiwei

图2 (GR)50 对AKT磷酸化水平的影响及调控AKT磷酸化对线粒体ROS水平、细胞凋亡水平的影响 Note： A. Detection of the expression levels of AKT and phosphorylated AKT in cells by Western blotting. B. Detection of the effect of SC79 on AKT and phosphorylated AKT levels in (GR)50-SK cells by Western blotting. C. Detection of the effect of SC79 on mitochondrial ROS level of (GR)50-SK cells by MitoSOX Red superoxide indicator staining. Red fluorescence represents intracellular ROS, and blue fluorescence represents DAPI-stained nucleus. Scale bar=10 μm. D. Detection of the effect of SC79 on cell apoptosis level by PI staining. Scale bar=100 μm.

Fig 2 Effect of (GR)50 on AKT phosphorylation level and the effect of regulating AKT phosphorylation on mitochondrial ROS level and cell apoptosis level