小鼠输卵管上皮类器官的构建及表型验证

吴其谦, 胡燕琴, 陈迦勒, 李牧辰, 赵有淦, 伍静文

Establishment and phenotype verification of mouse oviductal epithelial organoids

WU Qiqian, HU Yanqin, CHEN Jiale, LI Muchen, ZHAO Yougan, WU Jingwen

图1 WT小鼠和dKO小鼠输卵管上皮类器官形态学比较 Note： A. Different genotypes of mice detected by PCR. Triple (miR-34b/c+/- and miR-449-/-), miR-449-/-, and dKO (miR-34b/c-/- and miR-449-/-). B. Primary culture of the oviductal epithelial cells (×400, scale bar=50 μm). C. Identification of the purity of the isolated oviductal epithelial cells using immunofluorescent staining. Ac-α-tubulin for showing ciliated cells, PAX8 for showing secretory cells, ACTA2 for showing smooth muscle cells, and vimentin for showing fibroblasts (×400, scale bar=50 μm). D. Phase contrast images of organoids formation and growth from WT mice and dKO mice (×100, scale bar=200 μm). E. The numbers of the organoids with different sizes were counted after 8 d. F. The numbers of the organoids with different sizes were counted after 16 d. G. The total numbers of organoids were counted after 8 d and 16 d. ①P=0.002, ②P=0.007, ③P=0.004, ④P=0.006, ⑤P=0.001, ⑥P=0.011, ⑦P=0.013, compared with the WT group.

Fig 1 Morphological comparison of oviductal epithelial organoids from WT mice and dKO mice