小鼠输卵管上皮类器官的构建及表型验证

吴其谦, 胡燕琴, 陈迦勒, 李牧辰, 赵有淦, 伍静文

Establishment and phenotype verification of mouse oviductal epithelial organoids

WU Qiqian, HU Yanqin, CHEN Jiale, LI Muchen, ZHAO Yougan, WU Jingwen

图4 输卵管上皮类器官中纤毛细胞和分泌细胞的微观结构 Note： A. Transmission electron microscopic images of the ciliated cells of oviductal epithelial organoids of WT mice and dKO mice after 2 months of culture (×10 000, scale bar=1 μm). The images on the right are the magnification ones in the dotted box on the left (×25 000, scale bar=500 nm). The white line showing the arrangement of the basal bodies, and the white arrows showing multiple disorganized centrioles. B. Transmission electron microscopic images of the secretory cells of oviductal epithelial organoids of WT mice and dKO mice after 2 months of culture (×10 000, scale bar=1 μm). The images on the right are the magnification ones in the dotted box on the left (×25 000, scale bar=500 nm). The white arrows showing the exocytosis granules by the secretory cells.

Fig 4 Microstructures of ciliated cells and secretory cells in the oviductal epithelial organoids