小鼠输卵管上皮类器官的构建及表型验证

吴其谦, 胡燕琴, 陈迦勒, 李牧辰, 赵有淦, 伍静文

Establishment and phenotype verification of mouse oviductal epithelial organoids

WU Qiqian, HU Yanqin, CHEN Jiale, LI Muchen, ZHAO Yougan, WU Jingwen

图6 纤毛细胞与分泌细胞相关基因在输卵管上皮类器官中的表达 Note： A. Expression and localization of Ac-α-tubulin, FOXJ1, and PAX8 in the oviductal tissues and oviductal epithelial organoids of WT mice and dKO mice using immunohistochemical staining (×400, scale bar=50 μm). B. The expression levels of ciliated cell- and secretory cell-related genes of the oviductal epithelial organoids from WT mice and dKO mice detected by RT-qPCR. Tubb4a and Foxj1 for ciliated cells. Pax8 and Ovgp1 for secretory cells. C?E. Western blotting analysis of FOXJ1 and PAX8 protein levels of oviductal epithelial organoids from WT mice and dKO mice (C) with quantitative analysis of FOXJ1 (D) and PAX8 (E). ①P=0.002, ②P=0.001, ③P=0.000, ④P=0.018, compared with the WT group..

Fig 6 Expression levels of ciliated cell- and secretory cell-related genes in oviductal epithelial organoids