小鼠输卵管上皮类器官的构建及表型验证
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吴其谦, 胡燕琴, 陈迦勒, 李牧辰, 赵有淦, 伍静文
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Establishment and phenotype verification of mouse oviductal epithelial organoids
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WU Qiqian, HU Yanqin, CHEN Jiale, LI Muchen, ZHAO Yougan, WU Jingwen
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表1 实时荧光定量PCR引物序列
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Tab 1 Primer sequences in RT-qPCR
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| Primer | Sequence (5′→3′) |
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| Foxj1-F | AAGGAGGCAGAAATCCGGTG | | Foxj1-R | TTGTAGCCTCCCTTGTGCAG | | Pax8-F | CCCTTCGCCATAAAGCAGGA | | Pax8-R | AGCATGGGGAAAGGCATTGA | | Tubb4a-F | AACCCGGCACCATGGACTCTGT | | Tubb4a-R | TGCCTGCTCCGGATTGACCAAATA | | Ovgp1-F | TGGACCCCTTTCTTTGTACG | | Ovgp1-R | TGGACAGCAGTGTTTTCAGC | | Gapdh-F | TGGAAAGCTGTGGCGTGAT | | Gapdh-R | GGGTAGGAACACGGAAGGC |
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