SIRT2通过组蛋白H4K8去乳酸化修饰调控巨噬细胞趋化功能
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宋文汀, 陶悦, 潘艺, 莫茜, 曹清
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SIRT2 regulates macrophage chemotaxis by de-modifying histone H4K8 lactylation
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SONG Wenting, TAO Yue, PAN Yi, MO Xi, CAO Qing
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表1 RT-qPCR引物序列
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Tab 1 Primer sequences for real-time qPCR
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| Gene | Forward primer (5´→3´) | Reverse primer (5´→3´) |
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| HIF-1α | GAACGTCGAAAAGAAAAGTCTCG | CCTTATCAAGATGCGAACTCACA | | PFKL | GTACCTGGCGCTGGTATCTG | CCTCTCACACATGAAGTTCTCC | | LDHA | ATGGCAACTCTAAAFFATCAGC | CCAACCCCAACAACTGTAATCT | | SIRT1 | AGGCCACGGATAGGTCCATA | GTGGAGGTATTGTTTCCGGC | | SIRT2 | TGCGGAACTTATTCTCCCAGA | GAGAGCGAAAGTCGGGGAT | | SIRT3 | TGCTCATCAACCGGGACTTG | TTGTCTGGTCCATCAAGCCTA | | SIRT5 | CTCAAGATGCCAGCATCCCA | AGGAAGTGCCCACCACTAGA | | HDAC1 | CATCGCTGTGAATTGGGCTG | ACCCTCTGGTGATACTTTAGCAG | | HDAC2 | TCTGCTACTACTACGACGGTGA | TCATTTCTTCGGCAGTGGCT | | HDAC3 | CATGACGGTGTCCTTCCACA | CAGAGTCAGCTCCACACTGG | | GAPDH | TCTCCTCTGACTTCAACAGCGACA | CCCTGTTGCTGTAGCCAAATTCGT |
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