PRPS1 I72位点突变对急性淋巴细胞白血病耐药性的影响及其机制研究

崔芷嫣, 陈尧, 陶悦, 沈树红, 李慧

Effects of PRPS1 I72 mutations on drug resistance in acute lymphoblastic leukemia and its mechanisms

CUI Zhiyan, CHEN Yao, TAO Yue, SHEN Shuhong, LI Hui

图1 PRPS1 位点突变对293T细胞巯嘌呤类药物敏感性的影响 Note： A. Protein expression of PRPS1 mutants in 293T cells by Western blotting. His-PRPS1 represents the PRPS1 fusion protein with His label, that is, the exogenous PRPS1 protein. B. Detection of the drug sensitivities of PRPS1 mutations to 6-MP in 293T cells by drug sensitivity experiment. ①P=0.000, ②P=1.000, ③P=0.632, compared with 293T cells transfected with pGV303 PRPS1 WT plasmid. C. Detection of the drug sensitivities of PRPS1 mutations to 6-TG in 293T cells by drug sensitivity experiment. ①P=0.000, ②P=0.135, ③P=0.291, compared with 293T cells transfected with pGV303 PRPS1 WT plasmid.

Fig 1 Effect of mutations at PRPS1 locus on the sensitivity of thiopurine chemotherapy drugs in 293T cells