利用CRISPR/Cas9靶向敲除Piezo1基因对小鼠间充质干细胞成骨分化的影响研究

高昕, 杨屹羚, 黄湘如, 代庆刚, 江凌勇

Effect of Piezo1 on osteogenic differentiation of mouse bone marrow mesenchymal stem cells C3H10T1/2 based on CRISPR/Cas9

GAO Xin, YANG Yiling, HUANG Xiangru, DAI Qinggang, JIANG Lingyong

图2 Piezo1 基因敲除细胞系的成功构建 Note： A. PCR identification of CPK. M—Marker; Lane 1—negative control; Lane 2—wild cell; Lane 3—CPK. B. Base sequence of exon 4 of CON group cells and CPK group cells. C. qPCR results of Piezo1 expression. D. Immunofluorescence staining in CON group and CPK group.

Fig 2 Successful construction of Piezo1-knockout cell line