利用CRISPR/Cas9靶向敲除Piezo1基因对小鼠间充质干细胞成骨分化的影响研究

高昕, 杨屹羚, 黄湘如, 代庆刚, 江凌勇

Effect of Piezo1 on osteogenic differentiation of mouse bone marrow mesenchymal stem cells C3H10T1/2 based on CRISPR/Cas9

GAO Xin, YANG Yiling, HUANG Xiangru, DAI Qinggang, JIANG Lingyong

图4 敲除 Piezo1 基因对C3H10T1/2细胞成骨分化的影响 Note： A. ALP staining of CON group and CPK group after osteogenic differentiation induction in vitro. B. Alizarin red staining of CON group and CPK group after osteogenic differentiation induction in vitro. C. qPCR results of osteogenetic-related genes in CON group and CPK group.

Fig 4 Effect of Piezo1 knockout on the osteogenic differentiation of mouse bone marrow mesenchymal stem cells C3H10T1/2