利用CRISPR/Cas9靶向敲除Piezo1基因对小鼠间充质干细胞成骨分化的影响研究
高昕, 杨屹羚, 黄湘如, 代庆刚, 江凌勇

Effect of Piezo1 on osteogenic differentiation of mouse bone marrow mesenchymal stem cells C3H10T1/2 based on CRISPR/Cas9
GAO Xin, YANG Yiling, HUANG Xiangru, DAI Qinggang, JIANG Lingyong
表1 sgRNA寡核苷酸序列及引物序列
Tab 1 sgRNA oligonucleotide sequences and primers
NameSequences (5′→3′)
sgRNA-1GTTGAGACACCTTCACCCAA
sgRNA-5TCTGCCGGGCTTTCCTCGTG
Piezo1 forward primerATCAGCATTCCTGAATCACGGATG
Piezo1 reverse primerCTAGGGCTCTGTTACGCAGAG
Sequencing primerGGAAAGAATAGTAGACATAATAGC