七氟烷抑制新生小鼠前额叶皮质神经祖细胞向神经元分化发育
Sevoflurane inhibits the differentiation and development of neural progenitor cells into neurons in the prefrontal cortex of newborn mice
Note： A. Experimental procedures. The PFC cells acquired on postnatal day 9 (P9) from control (Ctrl) group, single anesthesia exposure (Sev1) group, and multiple anesthesia exposure (Sev3) group were prepared in single-cell suspension for 10x Genomics scRNA-seq. B. UMAP cluster showing PFC scRNA-seq results. C. Clustering visualization of RNA velocity analysis results of PFC cells. D. Bubble map of sorting gene markers in PFC cells. Bubble size represents the percentage of cells with the gene expressed in the population, and bubble colour represents the average value of the gene expressed in the population. E. Distribution of 3 groups of sample cells on the UMAP plot. F. Bar graphs of the percentages of cell types in 9 samples.
