间歇性禁食联合产热脂肪活化防治小鼠肥胖作用研究

吴凯敏, 麻静, 赵旭赟

Combined effects of intermittent fasting and thermogenic fat activation on the treatment and prevention of obesity in mice

WU Kaimin, MA Jing, ZHAO Xuyun

表1 RT-qPCR引物序列

Tab 1 Primer sequences for RT-qPCR

Gene Forward sequence (5′→3′) Reverse sequence (5′→3′) Rplp0 GAAACTGCTGCCTCACATCCG GCTGGCACAGTGACCTCACACG Ccl2 AGGTCCCTGTCATGCTTCTG TCTGGACCCATTCCTTCTTG Ccl5 TGCCCACGTCAAGGAGTATTT TTCTCTGGGTTGGCACACACT Il-1b GAAATGCCACCTTTTGACAGTG TGGATGCTCTCATCAGGACAG Il-6 AGTTGCCTTCTTGGGACTGA TCCACGATTTCCCAGAGAAC Ucp1 GGCATTCAGAGGCAAATCAGCT CAATGAACACTGCCACACCTC Cidea GCAGCCTGCAGGAACTTATCAGC GATCATGAAATGCGTGTTGTCC leptin AGCAGTGCCTATCCAGAA TGCCAGAGTCTGGTCCATCT adiponectin CAACTGAAGAGCTAGCTC CTTAGGACCAAGAAGACCTG Ppara AGAGCCCCATCTGTCCTCTC ACTGGTAGTCTGCAAAACCAAA Pparg CTGACCCAATGGTTGCTGAT GGTGGAGATGCAGGTTCTAC Ehhadh CAGATGAAGCACTCAAGCTTG ACCTTGGCAATGGCTTCTGCA Hmgcs2 GACATCAACTCCCTGTGCCTG GATGTCAGTGTTGCCTGAATC Cidec TCGACCTGTACAAGCTGAACCCT AGGTGCCAAGCAGCATGTGACC Srebp1c GATGTGCGAACTGGACACAG CATAGGGGGCGTCAAACAG Fasn GGAGGTGGTGATAGCCGGTAT TGGGTAATCCATAGAGCCCAG Scd1 GCTGGAGTACGTCTGGAGGAA TCCCGAAGAGGCAGGTGTAG Dgat2 GCGCTACTTCCGAGACTACTT GGGCCTTATGCCAGGAAACT Gck AGGAGGCCAGTGTAAAGATGT CTCCCAGGTCTAAGGAGAGAAA Pfkl TCCGCACCTACAACATCCAC GGCTGGGATGACACACATGA Hk2 TGATCGCCTGCTTATTCACGG AACCGCCTAGAAATCTCCAGA Pkm GCCGCCTGGACATTGACTC CCATGAGAGAAATTCAGCCGAG Glut1 TCAAACATGGAACCACCGCTA AAGAGGCCGACAGAGAAGGAA Glut4 GTGACTGGAACACTGGTCCTA CCAGCCACGTTGCATTGTAG Pepck CATATGCTGATCCTGGGCATAAC CAAACTTCATCCAGGCAATGTC G6Pase ACACCGACTACTACAGCAACAG CCTCGAAAGATAGCAAGAGTAG