细胞游离DNA在胆道癌诊断中的价值：一项meta分析

杨越, 何开举, 宗家豪, 杨自逸, 吴向嵩, 龚伟

Diagnostic value of cell-free DNA to biliary tract cancers: a meta-analysis

YANG Yue, HE Kaiju, ZONG Jiahao, YANG Ziyi, WU Xiangsong, GONG Wei

表2 纳入研究数据提取

Tab 2 Collected data of the included studies

Study	Sample	Method	Sample size/n	Cut off	TP/n	FP/n	FN/n	TN/n
HAN 2021^[25]	Bile	ddPCR	46	1 500 copies·mL^-1	20	0	22	4
HAN 2021^[25]	Plasma	ddPCR	20	60 copies·mL^-1	3	0	13	4
HE 2023^[26]	Bile	qPCR	188	NA	74	0	21	93
HE 2023^[26]	Bile	NGS	188	NA	80	2	15	91
HUA 2021^[27]	Serum	qPCR	158	403.65 ng·mL^-1	78	2	5	73
	Serum	qPCR	153	113.82 ng·mL^-1	83	0	0	70
	Serum	qPCR	158	364 ng·mL^-1	76	7	7	68
	Serum	qPCR	153	96 ng·mL^-1	83	0	0	70
KINUGASA 2018^[28]	Bile	NGS	43	NA	14	0	10	19
KUMARI 2019^[29]	Serum	qPCR	96	406.582 5 ng·mL^-1	48	5	12	31
	Serum	qPCR	96	1 128.429 ng·mL^-1	43	12	17	24
	Serum	qPCR	96	cfDNA integrity index: 0.356	47	7	13	29
	Serum	Methylated DNA Quantification Kit	96	Global DNA methylation: 0.713 5	33	18	27	18
KUMARI 2022^[30]	Serum	qPCR	75	251.2 ng·mL^-1	50	0	0	25
KUMARI 2017^[31]	Serum	qPCR	56	372.92 ng·mL^-1	30	0	4	22
KUMARI 2017^[31]	Serum	qPCR	51	218.55 ng·mL^-1	34	0	0	17
WANG 2021^[32]	Plasma	Low-coverage WGS	47	\|Z\|-score in UCAD test 2.32	26	2	3	16
WASENANG 2019^[33]	Serum	MSP	80	OPCML 3.24%‒50% methylation	32	4	8	36
	Serum	MSP	80	HOXD9 1.56%‒50% methylation	27	4	13	36
	Serum	MSP	80	HOXA9 1.56%‒50% methylation	19	15	21	25
	Serum	MSP	80	OPCML, HOXD9 both methylated	25	0	15	40
	Serum	MSP	80	OPCML, HOXA9 both methylated	12	1	28	39
	Serum	MSP	80	HOXA9, HOXD9 both methylated	10	1	30	39
	Serum	MSP	80	OPCML, HOXA9, HOXD9 ≥2 markers methylated	29	2	11	38
	Serum	MSP	80	OPCML, HOXA9, HOXD9 all methylated	9	0	31	40
WINTACHAI 2021^[34]	Plasma	qPCR	92	0.217 5 ng·µL^-1	55	1	7	29
WINTACHAI 2021^[34]	Plasma	qPCR	95	0.338 8 ng·µL^-1	51	14	11	19
MO 2020^[35]	Plasma	qPCR	85	18.06 ng·µL^-1	26	2	19	38