癌-睾丸抗原CT63在慢性髓系白血病中的作用及其机制

孔汝心, 周亚群, 魏婷宜, 雷鸣

Function and mechanism of cancer-testis antigen CT63 in chronic myeloid leukemia

KONG Ruxin, ZHOU Yaqun, WEI Tingyi, LEI Ming

图1 CT63 敲降后K562细胞增殖能力变化 Note： A. Kaplan-Meier survival curves of high and low expression of CT63 in myeloid leukemia patients. B. qRT-PCR analysis of CT63 knockdown efficiency in K562 cells. C. Western blotting analysis of CT63 levels in stable CT63 knockdown cell lines. D. Proliferation of stable shCtrl and CT63 knockdown cell lines assessed by live cell image assay. E. Live cell image analysis of proliferation rate of K562 after down-regulation of CT63 protein. F. Cell doubling time of K562 after down-regulation of CT63 protein. G. CCK-8 proliferation assay of indicated K562 cells. ①P=0.004, ②P=0.001, ③P=0.004, ④P=0.004, ⑤P=0.001, ⑥P=0.003, ⑦P=0.017, ⑧P=0.095, ⑨P=0.463, ⑩P=0.145, compared with the shCtrl group.

Fig 1 Proliferation of K562 cells after down-regulation of CT63