癌-睾丸抗原CT63在慢性髓系白血病中的作用及其机制

孔汝心, 周亚群, 魏婷宜, 雷鸣

Function and mechanism of cancer-testis antigen CT63 in chronic myeloid leukemia

KONG Ruxin, ZHOU Yaqun, WEI Tingyi, LEI Ming

图4 敲降 CT63 对K562细胞向单核/巨噬细胞分化的影响 Note： A, B, E. Immunofluorescence staining of CT63, CD11b, and CD14 after 48 h of PMA exposure in K562 cells. C, D, F. Relative fluorescence density of immunofluorescence staining. G. Western blotting analysis of CT63, CD11b and CD14 in K562 cells after 48 h of PMA exposure. H. CD11b positive cells of CT63-knockdown K562 cells and control after 48 h of PMA exposure. I. The percentage of CD11b positive cells. J. Immunofluorescence staining for CD11b and CD14 in tumor tissues. ①P=0.007, ②P=0.001, ③P=0.024, ④P=0.021, compared with the shCtrl group.

Fig 4 Effect of knockdown of CT63 on the differentiation of K562 cells into monocytes/macrophages